Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15 cells | Porphyromonas gingivalis |
into the pQE30 vector for transformation of Escherichia coli M15 cells | Porphyromonas gingivalis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, with 100 mM Ches (pH 9.0), 1.1 M potassium/sodium tartrate, 200 mM lithium sulfate | Porphyromonas gingivalis |
the structure of the prolyl tripeptidyl aminopeptidase complexed with the inhibitor is determined at 2.2 A resolution, the structure of the mutant E636A at 2.0 A, and in complex with the inhibitor at 1.95 A resolution | Porphyromonas gingivalis |
Protein Variants | Comment | Organism |
---|---|---|
E205A | mutant for examination of the function of the glutamate residue in substrate recognition, mutant expressed in inclusion bodies | Porphyromonas gingivalis |
E205Q | mutant for examination of the function of the glutamate residue in substrate recognition, mutant expressed in soluble form, no activity detected | Porphyromonas gingivalis |
E636A | reduced activity | Porphyromonas gingivalis |
E636A | mutant for examination of the function of the glutamate residue in substrate recognition | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala-Ile-pyrrolidin-2-yl boronic acid | - |
Porphyromonas gingivalis | |
Ala-Ile-pyrrolidine-2-yl boronic acid | - |
Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Gly-Ala-Pro-2-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
0.17 | - |
Ala-Phe-Pro-beta-naphthylamide | E636A mutant | Porphyromonas gingivalis | |
0.38 | - |
Gly-Ala-Pro-2-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
0.38 | - |
Gly-Ala-Pro-beta-naphthylamide | prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues | Porphyromonas gingivalis | |
0.42 | - |
Gly-Ala-Pro-2-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
0.42 | - |
Gly-Ala-Pro-beta-naphthylamide | prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues | Porphyromonas gingivalis | |
0.85 | - |
Gly-Ala-Pro-2-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
0.85 | - |
Ala-Phe-Pro-beta-naphthylamide | E636A mutant | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79390 | - |
- |
Porphyromonas gingivalis |
79390 | - |
calculated from amino acid sequence | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | Q7MUW6 | - |
- |
Purification (Comment) | Organism |
---|---|
Toyoperal HW650C chromatography and DEAE-Toyopearl column chromatography | Porphyromonas gingivalis |
using a Toyopearl HW650C and a DEAE-Toyopearl column | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Phe-Pro-2-naphthylamide + H2O | - |
Porphyromonas gingivalis | Ala-Phe-Pro + 2-naphthylamine | - |
? | |
Ala-Phe-Pro-beta-naphthylamide + H2O | - |
Porphyromonas gingivalis | Ala-Phe-Pro + beta-naphthylamine | - |
? | |
Gly-Ala-Pro-2-naphthylamide + H2O | - |
Porphyromonas gingivalis | Gly-Ala-Pro + 2-naphthylamine | - |
? | |
Gly-Ala-Pro-beta-naphthylamide + H2O | - |
Porphyromonas gingivalis | Gly-Ala-Pro + beta-naphthylamine | - |
? | |
Z-Gly-Ala-Pro-2-naphthylamide + H2O | - |
Porphyromonas gingivalis | Z-Gly-Ala-Pro + 2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
prolyl tripeptidyl aminopeptidase | - |
Porphyromonas gingivalis |
PTP | - |
Porphyromonas gingivalis |
PTP39 | - |
Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Porphyromonas gingivalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.65 | - |
Gly-Ala-Pro-2-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
2.65 | - |
Gly-Ala-Pro-beta-naphthylamide | prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues | Porphyromonas gingivalis | |
6.8 | - |
Gly-Ala-Pro-2-naphthylamide | mutant enzyme E636A, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
6.8 | - |
Ala-Phe-Pro-beta-naphthylamide | E636A mutant | Porphyromonas gingivalis | |
354 | - |
Gly-Ala-Pro-2-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
354 | - |
Gly-Ala-Pro-beta-naphthylamide | prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues | Porphyromonas gingivalis | |
511 | - |
Gly-Ala-Pro-2-naphthylamide | wild type enzyme, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
511 | - |
Ala-Phe-Pro-beta-naphthylamide | E636A mutant | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
activity assay | Porphyromonas gingivalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000881 | - |
Ala-Ile-pyrrolidin-2-yl boronic acid | wild type enzyme, using Gly-Ala-Pro-2-naphtylamide as a substrate, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
0.0000881 | - |
Ala-Ile-pyrrolidin-2-yl boronic acid | prolyl tripeptidyl aminopeptidase with an N-terminal truncation of 38 residues | Porphyromonas gingivalis | |
0.0488 | - |
Ala-Ile-pyrrolidin-2-yl boronic acid | mutant enzyme E636A, using Gly-Ala-Pro-2-naphtylamide as a substrate, in 20 mM Tris-HCl (pH 7.0), at 37°C | Porphyromonas gingivalis | |
0.0488 | - |
Ala-Ile-pyrrolidin-2-yl boronic acid | mutant E636A | Porphyromonas gingivalis |